Abstract:

The process of polysaccharide extraction from Sphacelotheca sorghi (Link) Clint was investigated in this study. The effects of extraction number and temperature, length of extraction time, and material/liquid ratio on polysaccharide yield were explored, and the optimization of the four process conciliations was achieved by orthogonal array design method based on single factor experiments. Extraction temperature was found to have the most important effect on polysaccharide yield, sequentially followed by material/liquid ratio, extraction number and length of extraction time. The optimal extraction process for polysaccharides was Sphacelotheca sorghi (Link) Clint was extracted three times for 2 h each time with a 20-fold volume of water in 80 ℃ water bath, and the resulting yield of crude water-soluble polysaccharides was approximately 7%. The purification of the crude product obtained was performed by using tannic acid and activated carbon together, and as a result, its purity was increased to 75.63%.

Key words: Sphacelotheca sorghi (Link) Clint, polysaccharide, extraction